The German cockroach (Blattella germanica) is the most widely distributed Blattaria flat varieties, and closely related to human life. Today, along with the global climate warming, the acceleration of urbanization process, ecological changes ceaselessly, and Blattella germanica breeding force strong, and the intimacy and has migration and dispersal habits, especially pesticide abuse caused by the resistance, Blattella germanica has become the world's most extensive and most difficult to manage urban health beneficial insects. To stop the biological characteristics of Blattella germanica and resistant population characteristics research, control urban Blattella germanica population change of discipline, and drug-resistance of Blattella germanica in to discuss the biological characteristics of the population characteristics, we formulate the stop war for control of scientific and effective strategy to provide the theory basis, has practical application. All research is divided into three departments: Department the first factor for the development of the German cockroach, Blattella germanica development and the breeding ways to change: the stop in the artificial weather box, separation set of 16 degrees, 18 degrees, 22 degrees C, 26 DEG, 30 and 34 degrees Celsius six temperature gradient, temperature of / 0. 5 DEG C, photoperiod LD1212h, light intensity of illumination for 4000lux, absolute humidity is 65 / 5)% RH, indecent observes the development of German cockroach, Blattella germanica development situation, according to the useful temperature of rail, and calculation of Blattella germanica in different insect state useful temperature (k) and the development of end-point temperature (DEG C). In the light period is LD168h, LD1212h, LD816h, (the intensity of light is 4000 Lux), and LD024h (Quan Yinyu), the temperature is set to 26 degrees Celsius, the temperature difference is / 0. 5 DEG C, the absolute humidity is the 65 / 5)% RH created under the premise, not Yacha different photoperiod on nymphal development development, breeding and life history of rhythm. Set no water and food, there is no water premise food three nutrient dilemma, the survival rate of observation nymphs. The development duration was the longest in all stages and the whole generation at 18 degrees C, and the later stage was 20. 67 / 2. 03D; egg stage 45. 33 / 0. 33d; 109 nymph. 67 / 2. 91d; whole generation 175. 67 / 2. 91d. The development duration was the shortest in all stages and the whole generation at 34 degrees C, and the later stage was 4. 67 / 0. 33d; egg stage 10. 33 / 0. 67D; 34 nymph. 67 / 0. 88D; whole generation 49. 67 / 1. 76d. The German cockroach egg development end temperature is 14. 74 degrees, the temperature for 184 useful. 8 - degree; the end temperature is 9 nymphal development. 95 degrees, the temperature for 732 useful. 7 days, the end of the development of the late spawning temperature is 13. 99 degrees, the temperature for 85 useful. 3 days and degrees; the development of the whole generation terminal temperature is 11. 26 C for 1031 useful temperature. 1 days and degrees. Temperature of 26 degrees Celsius, the nymph development speed LD168h > LD1212h > H LD816 > LD024h, nymphal survival rate, single female egg hatching clamp were with light when the number of cuts reduce, but differences in LD1212h LD168h no statistical significance; females than is with light when the number of a significant reduction in the lower; population trend index (I) in the LD168h most Nianye, 13. 22, while LD024h is the smallest, only 2. 31. Different strains of German cockroach nymphs three dilemma under the premise of the survival time is not the same, no food anhydrous premise, survival time is the shortest, sensitive strains of the 2D began to appear death, on the 5th day survival rate only 26. 7%, the longest survival time is 7d. The scene of the Department of product line 2D beginning of the death of the death, the first 5D survival rate was 90%, the longest survival time was 14D. Under the premise of fresh water, the sensitive strain of the first 2D of the beginning of the emergence of death, the first 5D survival rate was 50%, the longest survival time 12D. The scene of the Department of the same 2D is the beginning of the emergence of the death of the first 5D, the survival rate of 93. 3%, the longest survival time 19d. There is no food of water under the premise of sensitive information in fourth days beginning to emerge in death, and 53 for 20d. 3% survive, the longest survival time is 40d. And the scene of the Department of the Department of 20d is still 66. 7% survive, the longest survival time is 59d. Conclusions temperature and photoperiod as the main ecological factor, in Blattella germanica development, development and growth plays an important role in the process; under suitable temperature, according to the LD168h light is German cockroach nymphs development development more suitable light illumination, permanent sunshine types of Blattella germanica photoperiodic response pattern, the nymphal stage in life history occupied the location of the main; the German cockroach, Blattella germanica field lines on the situation comply with stronger. Community characteristic way of the second Department of Blattella germanica resistant German cockroach, Blattella germanica field strains (R) collected from the suburbs of Jinan four sampling points, separation mark for JN1, JN2, jn4 and jn4 and bioassay by membrane method for determination of the sensitivity of nymphs of acephate and deltamethrin, and calculate the two drugs mixed with the CO toxicity coefficient (CTC); biochemical method take acetylcholinesterase (AChE) kit and glutathione s transfer enzyme (GSTs) test kit, the determination of acephate and deltamethrin mixed with the activity of ache and GSTs change. The results of different collection points JN1, was JN2, JN3 and JN4 of Blattella germanica nymph KT50 is separated into 56. 96 min, 49. 85 min, 75. 78 min and 35. 63min, the resistance coefficient (R/S) was separated into 3. 1, 2. 8, 4. 1 and 1. 9; deltamethrin collection points on JN1, JN2, JN3 and JN4 differences of Blattella germanica nymph KT50 is separated into 20. 0841 min, 13. 56 min, 24. 03 min and 10. 65min, the resistance coefficient (R/S) was separated into 5. 5, 3. 8, 6. 7 and 3. 0; the and JN2 populations of the JN1 and CTC populations were separated by two. 5 and 130. The results also showed that the activity of 9; AChE and GSTs are associated with sensitivity, and enzyme activity is higher than that of adult nymph. Deltamethrin mixed with acephate (110), 70min AChE from the beginning of the 0 activities. 8248 (U/mgprot) down to 0. 4039 (U/mgprot); GSTs gas from the beginning of 290. 004 (U/mgprot) rose to 466. 752 (U/mgprot). Conclusion unified regional divergence field strains of Blattella germanica to insecticide sensitivity differences, showing significant regional population; different mechanism of action of pesticides is reasonable mixing can effectively improve the insecticidal effect. Research method of Blattella germanica in ache and GSTs activity were higher than that of the adult, this interpretation of nymphs than adults to insecticide sensitivity of absolute to low, which from Blattella germanica in physiological and biochemical aspects illustrates the German cockroach nymphs in the life history has an important position. Remind in real control tasks, to grasp the nymph especially. The third Department of German cockroach master strategic consultation through the process research of Blattella germanica population characteristics and drug resistance of the population characteristics of that population density of Blattella germanica control strategy should adopt comprehensive measures of physical control, ecological control, biological control and chemical control. Especially in the application of chemical pesticides, should according to the ecological characteristics of Blattella germanica, exercise habits and its and the relationship, as far as possible to reduce pesticide target area, stop insecticides mixed with the application and key habitat use pesticides ecological selective use of technology research, to reduction of purification. 目录: |
