作 者：JIANG Keyong , SUN Shujuan , LIU Mei , WANG Baojie, MENG Xiaolin, WANG Lei 1 R＆D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 Graduate University of Chinese Academy of Sciences, BeoTng 100049, China 机构地区：[1]R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Graduate University of Chinese Academy of Sciences, Beo'ing 100049, China 出 处：《中国海洋湖沼学报：英文版》2017年第1期118-127,共10页Chinese Journal of Oceanology and Limnology 基 金：Supported by the National Natural Science Foundation of China （No. 41206144） and the National High Technology Research and Development Program of China （863 Program） （No. 2017AA 100805） 摘 要：安培脱氨基酶催化安培的变换进淘气鬼和氨。在现在的学习，日语论文题目，从日语的骨胳的肌肉的 AMPD1 的全身的 cDNA 挣扎 Paralichthys olivaceus 被克隆并且描绘。2 526 bp cDNA 包含 78 bp， 237 bp 的 3-UTR 和 2 211 bp 的一个开的读物框架(ORF ) 的 5-UTR，日语毕业论文，它编码 736 氨基酸的蛋白质。预言的蛋白质包含一个高度保存的安培脱氨基酶主题(SLSTDDP ) 和一个 ATP 有约束力的地点序列(EPLMEEYAIAAQVFK ) 。种系发生的略论证明 AMPD1 和 AMPD3 基因从一样的分支发源，但是离 AMPD2 基因 evolutionarily 远。RT-PCR 证明 flounder AMPD1 基因仅仅在骨胳的肌肉被表示。QRT-PCR 略论揭示了一统计上重要在成年肌肉的 AMPD1 mRNA 的 2.54 褶层高水平(750 浥条汧瑵湩湩 ? 挠湯慣慮慶楬??? 潰祬楲潢湩獯湩捩瀠汯特扩 ' 椰熌π谵?????? € ????? ″ ? 洠乒 ??? ???????? ㄠ′ ???? 吗？？AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5＇-UTR of 78 bp, a 3＇-UTR of 237 bp and an open reading frame （ORF） of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif （SLSTDDP） and an ATP-binding site sequence （EPLMEEYAIAAQVFK）. Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle （750±40 g） compared with juvenile muscle （7.5±2 g） （P〈0.05）. HPLC analysis showed that the IMP content in adult muscle （3.35±0.21 mg/g） was also statistically significantly higher than in juvenile muscle （1.08±0.04 mg/g） （P〈0.05）. There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals. 关 键 词：全长cDNA 腺苷脱氨酶 MRNA表达 AMPD1基因 克隆 牙鲆 日本 RT-PCR略论 分 类 号：S855.3[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医] Q55[生物学—生物化学] |
