| 摘要:（摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 2017一2017年间对新疆石河子、玛纳斯县、呼图壁县、吉木萨尔县、和硕县等15个加工番茄主产区停止了深刻查询拜访，发明田间番茄除表示花叶、蕨叶等罕见病毒病症状外，还有一些植株表示为顶端全体黄化，而中下部叶片表示正常，俄语论文，与通俗黄化症状纷歧致，田间呈随机散布状，病发率在2%一50%之间，分歧地域的病发率和病发水平有必定差别。病发率与种类、栽培措施和灌水措施没有显著的关系。经由过程ICP一AES法对加工番茄顶端黄化样品9种元素含量停止测定，成果显示：顶端黄化植株中Mg、Mn元素的含量略微低于正常植株，而B、K、Mo、Na、Zn的含量则显著高于统一地块的正常植株，Fe、Ca元素的含量在黄化植株中和正常植株中则没有显著差别。根本明白了加工番茄顶端黄化并不是由心理性缺素惹起。经由过程对田间样品病毒粒子粗提液的电镜不雅察，发明有多种病毒粒子存在。直接酶联免疫吸附法对30个田间样品停止检测的成果注解CMV的检测率为100%，TMV、ToMV、BBWV的检测率分离为66。7%、96。7%、70。0%。运用TMV、ToMV、CMV、PVS、PVM等特异性引物对加工番茄顶端黄化样品停止RT一PCR扩增，俄语论文范文，获得了响应的目的条带，这与ELISA检测成果根本分歧，加工番茄顶端黄化样品中有多种病毒存在。初步以为加工番茄顶端黄化能够与这些病毒有必定关系，但病因仍不清晰。将PVM特异性引物PVMR/F扩增出的目的片断停止克隆并测序，经由与GENEBANK中其他已报导的PVM部门序列比对，成果显示：RT一PCR扩增出的目的条带是PVM的一段基因，有1198个核苷酸构成，包括完全的PVMCP基因(记为PVM一XJ)。与基因库中的其他代表分别物比拟，核苷酸序列与中国杭州(AJ437481)、美国(AF023877)、波兰(AY311394)、伊朗(EF397743)、拉脱维亚(GQ496609)、俄国(NC001361)、德国(X57440)、意年夜利(X85114)和加拿年夜(EF063383、EF063384、EF063387)地域分别物的同源性为74。0%一95。8%。PVM一XJ与俄国的登录号为NC001361的亲缘关系比来，与加拿年夜的EF063387关系最远。初次在加工番茄上用份子检测办法判定到了PVM。 Abstract: 2017 - 2017 years of Shihezi, Manas County, Hutubi County, Jimsar County, Heshuo county 15 processing tomato production areas stopped profound query visit, invention field tomatoes in the mosaic, fern leaves and other rare virus disease symptoms, and some plants said as the top all yellow, and lower leaves normal, and popular yellowing symptoms are inconsistent, the field was randomly scattered, incidence of a disease is between 2% - 50%, regional differences of disease hair rate and level of a certain differences. There was no significant relationship between the incidence and types, the cultivation methods and irrigation methods. Through process in ICP AES method of top yellowing of processing tomato samples of 9 kinds of elements content determination results show: Top chlorosis in Mg and Mn content is slightly lower than normal plants, and B, K, Mo, Na, Zn content was significantly higher than that of normal plant plots unified, the content of elements such as Fe, CA in etiolated plants and normal plants is not significant difference. Understand the basic processing tomato top yellowing and not by the psychological deficiency caused. Through the process of electron microscopy of field samples of virus particles of crude extracts was invented, a variety of virus. The results of 30 field samples were detected by direct enzyme linked immunosorbent assay, and the detection rate of CMV was 100%. The detection rate of TMV, ToMV and BBWV was 66. 7%, 96. 7%, 70. 0%. Application of TMV, ToMV, CMV, PVS, PVM and specific primers on top yellowing of processing tomato samples stop RT - PCR amplification, obtained the response to strip. The ELISA results fundamental differences, the top yellowing of processing tomato samples have exists many kinds of virus. The preliminary conclusion is that the top yellowing of processing tomato can be certain relationship with these viruses, but the etiology is still not clear. Cloning and sequencing of the PVM specific primers PVMR/F amplified fragment, via with the genebank in other reported PVM Department sequence alignment results show: RT PCR amplified band is a piece of PVM gene, composed of 1198 nucleotides, including completely PVMCP gene (denoted as a PVM XJ). And other representatives of the gene pool, respectively were compared and nucleotide sequence and China (aj437481), United States (af023877), Poland (ay311394), Iran (ef397743), Latvia (gq496609), Russia (nc001361), Germany (x57440), Italy (x85114) and Canada (ef063383, EF063384, ef063387) region respectively of homology to 74. 0% a 95. 8%. PVM a XJ and Russia's accession number for the NC001361 phylogenetic relationship than, with the addition of the eve of the EF063387 relationship is the most distant. The first time in the processing of tomato using molecular detection method to determine the PVM. 目录: |
