The German cockroach (Blattella germanica) is one of the beneficial insects in the world, China is the largest indoor health insects see. Current of Blattella germanica control in the chemical control based, large amount without reasonable application of chemical pesticides has lead to Blattella germanica and gradually strengthen the response to insecticides resistance, thereby increasing the difficulty of control, also add to the eve of the prevention and control of the cost of. A collection of pheromones of Blattella germanica set preface material damage. With the pheromone difference is that it can contain all of nymphs and adults, the individual trapping effect. Application set this feature of pheromone, a joint application with insecticide, Blattella germanica poly and kill it, or for bait in reducing some stomach poisons on target insect repellent, will progress control consequences. International Research on Blattella germanica aggregation pheromone although a lot of, but are concentrated in biological activity and chemical composition analysis, and currently does not familiar with differences; and on the aggregation pheromone of biological decomposition and its regulation mechanism study, currently there is no any reports. Effect of illumination; combined with the origin based on the above background, this subject in Germany small forging as the research object, through the process of olfactory action monitor on the body surface and stool collection activity to carry on the research to certain information, and then put out a collection of information useful ingredients, through the process of gas mass spectrometer (GC MS) certainly active material chemical component and content on the basis of the research on brain suboesophageal ganglion (BR SOG), juvenile hormone (JH), molting hormone (MH) and differences on the premise of Blattella germanica set pheromone biological decomposition to Germany certainly agglutination pheromone biological decomposition of regulation factor and illustrate its regulation mechanism. Further by RTPCR technique of gastric pharyngeal side neuropeptide (AST) cDNA cloning and sequence determination, from molecular biology degree of pheromone biodegradable regulating factors and regulation mechanism of stop deep research, in order to for new cockroach prevention and control strategy in supply theoretical basis. The main research results of this project are as follows: 1 the biological activity of the German small profit collection of 1 is measured. 1 in vivo induced source activity was detected in the collection of a German small must nymphs, adult females and adult male the test insects in vivo induced source, of the test insects has trapping effect is obvious and retention effect. One of the weakest trapping activity of male adults, nymphs and adult females were had strong activity. The in vivo induced source of nymphs nymphs and female adults set probation and probation detention are strong, but the effect on male adult was relatively weak, the set index (AI) was 0.79, 0 and 6 were isolated and 0.58, the retention index (RI) was separated into 0 3 stone, 0 stone 6 and 0.52 in female; adult worm induced source of 3 kinds of test set with 0 "cooperation and retention effect are very strong, AI is separated into 0.79, 0.76 and 0.77, the RI is separated into 0 7 stone, 0 stone 4 and 0 stone 5; living adult male attractant to male adults are set probation and probation detention weak, but for t nymphs and female adult probation are absolutely strong, AI is separated into 0.33, 0.66 and 0.70, 0.41, 0 RI is separated into 0 and 0 stone stone 6. The annotation of the achievement, German cockroach regardless of age and gender can directly from the wall of the release of aggregation pheromone, and collection of information element by the LED components and retention components constitute. 1.2 fecal collection activity of the collection activity is also the main origin of the collection of information, the 3 types of test insect feces collection activity is greater than the response of the living source. A set of activity and retention of nymph feces was the strongest, the weakest activity of male adult feces. Nymph feces induced source in female adults of the most powerful role, AI is as high as $0 1, 3 & test insects retained activity are very high (RI are larger to 0.76); female adult feces induced source of 3 kinds of test insect attractant activity are higher (AJ are larger than 0.70), to male adults retentive activity is definitely weaker (* 1 to 0. 59 male adult feces of the 3 types of test insects are not the difference between the effects of the 0 Stone 8, 0.59 and 0.66, RI separation of 0 stone 6, 0 stone 0 and 0 stone 4. 1.3 German Hwan small nymphs of skin and fecal crude extract of nymphs of the active set detection "Blattella mite nymphs of skin and fecal extract of nymphs have significant direct effect and retention of activity and fecal extracts guided activity and retention of activity were significantly higher than that of the surface extract. Hexane and dichloromethane are useful extraction agent of pheromone. Consequences of 1.4 German small sickle fecal crude extract and bait mixed after insecticidal Germany small forging nymphs of the crude extracts of the feces can perhaps significant progress a by bait insecticidal consequences, down test insect bait repellent reaction, extends the application time of bait. The death rate of the treatment area was much higher than that of the control area. . German small sickle set pheromone extraction and active components and contents determination with gas chromatography (GC) amiable mass spectrometry (GC - MS) combined techniques used in determination of the hexane extract of Blattella butterfly surface dichloromethane extracts and feces, extracts of surface active components are all on the surface of the skin excretion of hydrocarbons, include straight chain alkanes, methyl in an odd number of carbon atoms of methyl branched and dimethyl branched from 27 to 31 alkyl carbon. Fecal extract female adults and nymphs of the GCMS spectrum. 目录: |
