方斑东风螺海南与泰国群体杂交试验及其五个群体遗传多样性探讨Studies on the cross test of and Thailand and the genetic diversity of five populations in the east of the and the Hainan 摘要:（摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 本论文以方斑春风螺[Babylonia areolata（Lamarck）]为研究对象，分离以海南群体（HH）、泰国群体（TT）、汕头群体（SS）、湛江群体（ZZ）和海南♂×泰国♀（HT）群体为资料，采取RAPD和SSR份子标志技巧对方斑春风螺5个分歧群体的遗传多样性停止剖析比拟；丈量和剖析方斑春风螺海南群体和泰国群体的群体内自交子一代和群体间杂交子一代苗种发展性状差别。重要试验成果以下 （1）方斑春风螺海南群体和泰国群体自交和杂交子一代的发展丈量本研究运用14项丈量目标对方斑春风螺4个子一代群体停止评价，成果注解各子一代群体在各性状上表示出的优势其实不雷同，泰国♂×海南♀杂交组的丈量数据在部门丈量目标上位于两自交组之间，泰语毕业论文，这解释杂交子一代融会了父本和母本的部门基因，表示出必定的杂交后果。在稚螺到成螺的养殖阶段，海南♂×泰国♀组体重的日均匀增加速度在4个组合中是最高的，这是具有临盆性意义的杂种优势。但杂交组合在绝年夜部门丈量项目上未表示出预期的杂种优势，海南♂×泰国♀杂交组的数据在有一半的丈量项目是排在最初。总的来讲，子一代群体在某一些性状上表示出优势，而在另外一些性状上又未能表示出优势，是以综合性状仍没法表示出优势，即杂交组合未显示出杂种优势。 （2）运用RAPD和SSR两种份子标志技巧，剖析方斑春风螺5个分歧群体的遗传多样性。试验共挑选出20条随机引物和7对SSR引物，对5个群体各30个个别的基因组DNA停止扩增，运用Popgen32软件剖析统计数据，用UPGMA法构建方斑春风螺分歧群体的体系产生树。 方斑春风螺5个群体的遗传多样性研究RAPD标志共取得237个反复性好且条带清楚的扩增位点，片断年夜小在100一2000bp之间，5个群体的多态位点比例在55。13％一70。26％之间；SSR标志共取得41个多态性好且条带清楚的等位基因，片断年夜小在100一400by之间，5个群体的PIC含量在0。5861一0。6599之间。SSR检测的不雅察杂合度Ho在0。6000一0。6905之间，希冀杂合度He在0。5885一0。6503之间。各群体的均匀不雅测杂合度到达了0。6560，希冀杂合度的值为0。6156，涌现杂合渡过剩景象，解释各群体处于杂合度很高的状况。两种办法的检测成果都注解5个群体都具有很高的遗传多样性，TT群体和HH群体的遗传多样性最高，SS群体和ZZ群体次之，HT群体最低。 运用SSR测得的群体间总的近交系数（Fst）为0。0349，成果注解群体间涌现了必定的分化，但与群体内的变异比拟，群体间的变异绝对较小。卡方磨练检测Hardy一Weinberg均衡时也发明，一切群体在P2位点都偏离了Hardy一Weinberg均衡，在其它6个位点处，则涌现部门群体偏离Hardy一Weinberg均衡状况。证实了群体遗传构造不屈衡。 运用两种办法盘算群体间的遗传间隔在0。0680一0。1817之间（RAPD）和0。0301一0。0960（SSR）之间。基于遗传间隔所构建的体系树注解，HH群体和SS群体遗传间隔最小，亲缘关系比来，起首聚类在一路，以后与ZZ群体聚类，再与HT群体聚在一路，最初与TT群体聚类在一路（RAPD成果）。5个群体共分为3个组，泰语论文范文，TT和HT群体的遗传间隔比来，最早聚为一组；SS群体和TT、HT群体的遗传间隔较近，聚在一路；HH和ZZ群体的遗传间隔较近，聚为第二组后再与前3个群体聚在一路（SSR成果）。 Abstract: The square spot spring screw [Babylonia areolata (Lamarck)] as the research object, separation to Hainan population (Hh), Thailand group (TT), Shantou group (SS), Zhanjiang group (zz) and Hainan (* Thailand female (HT) groups for data, take RAPD and SSR molecular marker skills other spot spring screw five differences between the groups of genetic diversity of comparative analysis, and Thai populations of measurement and analysis spot spring screw Hainan population groups within the self progeny and groups intermingled filial generation seed development traits difference. Below the important test results (1) square spot spring screw Hainan population and Thailand inbred population hybrids and generation of development measure this research application 14 measurement target other spot spring screw 4 sub generation group evaluation, results of annotation by sub generation groups in each trait that advantage actually not the same, Thai male X Hainan by hybridization group measurement data in the measurement objectives of the division is located in between two inbred group, which explained hybrids generation blend of male and female Department gene, showing certain hybrid consequences. In the juvenile snails to snail breeding stage, uniform Hainan male * Thailand female group, the body weight, increased speed in four combinations is the highest, which is with Heterosis for birth. But hybrid combinations in the vast majority of measure project has not shown the expected heterosis, Hainan male * Thailand female hybrid group data in half of the measurement project is a row in the first. Generally speaking, the children of a generation group in a number of traits expressed the advantage, and in addition to some traits and failed to show the advantage, is still unable to show the advantages of comprehensive traits, that is not to show the hybrid combination of heterosis. (2) using RAPD and SSR two kinds of molecular markers to analyze the genetic diversity of 5 different groups. Test selected 20 random primers and 7 pairs of SSR primers, the 5 groups of 30 individual genome DNA amplification, the application of Popgen32 software analysis of statistical data, using UPGMA method to construct the system of square spot spring snail. Five square spot spring snail populations genetic diversity study RAPD markers were obtained from 237 repeatability is good and a clear amplified loci and between 100 a 2-kb fragment, the percentage of polymorphic loci of the five populations in 55. 13% a 70. Between 26%; SSR markers were obtained 41 polymorphic good and the strip clear allele, fragments of the eve of the small in 100 between a PIC, 5 groups of 400by content in 0. 5861 a 0. Between 6599. The detection of SSR observation heterozygosity in 0 Ho. 6000 a 0. 6905, the expected heterozygosity of He in 0. 5885 a 0. Between 6503. The average observed heterozygosity of each population reached 0. 6560, the value of the expected heterozygosity was 0. 6156, the emergence of surplus surplus phenomenon, explain the high degree of heterozygosity of each group. Detection results of the two methods are annotated with a high genetic diversity of 5 groups, TT group and HH group, the highest genetic diversity, SS groups and ZZ groups, the HT group is the lowest. The total inbreeding coefficient (Fst) of the population measured by SSR was 0. 0349, the results of the annotation groups have a certain degree of differentiation, but compared with the variation within the population, the variation among groups is relatively small. Chi square test Weinberg a Hardy balance also found that all groups in the P2 loci have deviated from the Weinberg a Hardy equilibrium, at the other 6 loci, the emergence of a group of groups from the Weinberg equilibrium Hardy equilibrium. It is proved that the population genetic structure is not balanced. Application of the two methods to calculate the genetic distance between populations in 0. 0680 a 0. Between 1817 (RAPD) and 0. 0301 a 0. Between 0960 (SSR). Based on the phylogenetic tree constructed by genetic distance, the HH group and the SS group had the smallest genetic distance, genetic relationship ratio, first cluster in one way, after clustering with ZZ group, then together with the HT group in the first, and the first TT group clustering in the way (RAPD results). 5 groups were divided into 3 groups, TT and HT groups of the genetic interval ratio, the first to gather for a group; SS group and TT, HT group of genetic interval, together in the way; HH and ZZ groups of the genetic interval closer together, together with the second groups after the first 3 groups together (SSR results). 目录: |
