| （摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 德国小蠊（Blattella germanica）是世界性的最难管理的卫生益虫之一。为了有针对性地对德国小蠊停止有用防治，本文对德国小蠊的防治药剂停止了室内挑选，并研究了农药亚致逝世剂量对德国小蠊靶标酶活性的作用和食品和德国小蠊粪便粗提物对它的引导感化，德语论文范文，重要成果以下毒力测定成果注解，溴氰菊酯、氯氰菊酯、辛硫磷和敌敌畏处置后，德国小蠊5龄若虫的耐药性均高于其它龄期和牝牡成虫，溴氰菊酯、氯氰菊酯的KT50分离为14。16和24。60，辛硫磷和敌敌畏的LC50分离为827。26 mg/L和592。65 mg/L，与1龄比拟，其绝对耐药倍数分离为1。97、2。46、20。72、17。86，并且雄性成虫的耐药性均年夜于雌性成虫。混配实验成果注解，氯氰菊酯和辛硫磷按有用成份15混配后增效最显著，共毒系数为188。29。氯氰菊酯与敌敌畏（15）、溴氰菊酯和敌敌畏（15）都有较好的增效感化，共毒系数分离为152。05和130。94，而溴氰菊酯和辛硫磷按有用成份15配比后增效感化不显著，共毒系数为119。41。分歧龄期、分歧性别德国小蠊体内酶活性测定注解，对于羧酸酯酶和乙酰胆碱酯酶，跟着龄期的增长，德语论文范文，活性逐步下降且差别明显。雄成虫的羧酸酯酶活性显著年夜于雌成虫，为雌成虫的1。74倍，其差别明显，但乙酰胆碱酯酶活性差别不明显。对于谷胱甘肽S一转移酶，按每μmol/mgpro·min表达活性时，在德国小蠊1、3、5龄若虫及雌、雄成虫中，活性简直没有差别。但如果转化成μmol/虫·min表达，其活性跟着龄期的增长而增长。亚致逝世剂量对德国小蠊体内酶活性作用的测定成果注解经溴氰菊酯亚致逝世剂量LC15（5。90mg/L）处置后，羧酸酯酶活性在72h内逐步下降，比处置前下降了28。02%，乙酰胆碱酯酶活性在24h时略有降低，比处置前降低了19。79%，然后逐步下降，96h时降到最低；经辛硫磷亚致逝世剂量LC20（203。77 mg/L）处置后，羧酸酯酶活性在24h时最低，乙酰胆碱酯酶活性逐步下降，异样在96h时降到最低，比处置前下降了27。27%。谷胱甘肽S一转移酶活性在72h时降到最低，与处置前比拟分离下降了25。03%和20。23%，随后略有降低。在食品引导实验中，炒年夜豆粉对德国小蠊的引导后果最好，诱集率到达45。66%；喷鼻蕉次之，诱集率到达了25。83%；小白菜汁的引导后果较差，诱集率为16。94%；葡萄糖的诱集率最低，为11。67%。将炒年夜豆粉与喷鼻蕉与溴氰菊酯和辛硫磷配成毒饵，诱杀成果注解，喷鼻蕉所配制的毒饵诱杀后果最好。在德国小蠊粪便粗提物引导实验中，起首挑选出乙醇为最好萃取溶剂，粪便的乙醇提取物对德国小蠊的诱集成果注解，低龄若虫粪便的乙醇提取液对低龄若虫、高龄若虫、雌成虫、雄成虫的引导后果都是最好的，诱集率分离为39。72%、40。56%、38。33%和34。17%。在毒饵平分别参加必定量的低龄若虫和高龄若虫粪便的乙醇提取物时，其诱杀活性显著进步，低龄若虫的诱杀后果高于高龄若虫。 Abstract: The German cockroach (Blattella germanica) is one of the world's most difficult health pest management. In order to have for of Blattella germanica effective prevention and control, this paper of Blattella germanica control agents stopped indoor selection, and a discussion of the pesticide sub induced death dose of Blattella germanica target enzyme activity influence and food and Germany cockroach feces crude extract on its direct effect, important achievements of the following virulence determination results annotations, deltamethrin, chlorine, fenpropathrin, Phoxim and dichlorvos disposal, 5 instar larvae of Blattella germanica resistance were higher than other age and male and female adults, deltamethrin, cypermethrin kt50 of separation is 14. 16 and 24. 60, LC50 separation of Phoxim and dichlorvos was 827. 26 mg/L and 592. 65 mg/L, compared with 1 years of age, the absolute resistance ratio was 1. 97, 2. 46, 20. 72, 17. 86, and the resistance of male adults was greater than that of female adults. The experimental results of mixed notes, cypermethrin and phoxim in useful ingredients 15 after mixing efficiency most significantly, the CO toxicity coefficient was 188. 29. Cypermethrin and dichlorvos (15), deltamethrin and DDVP (15) has good synergistic effect, a total of Toxicity Coefficient separation for 152. 05 and 130. 94, while deltamethrin and phoxim in useful ingredients 15 ratio is not significant synergistic effect, the CO toxicity coefficient was 119. 41. Differences in age, gender differences and Blattella germanica in vivo enzyme activity determination of annotation, about carboxylesterase and acetylcholinesterase (AChE), along with the growth of the age, activity gradually decreased and the difference is obvious. The esterase activity of the male adult was significantly larger than that of the female, which was 1 of the female adult. 74 times, the difference was obvious, but the AChE activity was not obvious. On the glutathione s - transferase, according to the per mu mol/mgpro - min expression activity, in the German cockroach, Blattella germanica in 1, 3, 5 instar nymph and male and female adult activity almost no difference. But if converted into mol/ worm min expression, its activity with the growth of the age and growth. Determination results of the death note dose effect on the German cockroach activity by deltamethrin induced death of LC15 dose (5 sub. After treatment with 90mg/L, the esterase activity of the carboxylic acid esterase decreased gradually in the 72h, which was decreased by 28 compared with that before treatment. 02%, the AChE activity in 24h was slightly lower than that of the treatment before the reduction of 19. 79%, and then gradually decreased, 96h was reduced to the minimum; the death dose of the LC20 (203. 77 mg / L) after disposal, carboxylesterase activity at 24h minimum acetylcholinesterase activity decreased gradually, strange in 96 hours down to lower than before disposal dropped 27. 27%. Glutathione S transferase activity in 72h was reduced to a minimum, compared with the treatment before the separation decreased by 25. 03% and 20. 23%, followed by a slight decrease. In the food guide experiment, guide the consequences of fried Nianye soybean powder on Blattella germanica best, the trapping rate reached 45. 66% bananas; second, the trapping rate reached 25. 83%; poor guide consequences of Pakchoi juice, the trapping rate is 16. 94%; glucose trap was the lowest, 11. 67%. Fried Nianye flour and nasal spray bananas and deltamethrin and phoxim with bait, trap results annotations, spray nasal Manila in the preparation of the baiting result is best. In Blattella germanica feces extracts guide experiment, first select the ethanol as the best solvent extraction, fecal ethanol extract against the German cockroach, Blattella trap results annotations, nymph feces of ethanol extract of nymph, the elder nymph, adult female, male adults guide consequences are the best trap isolation rate was 39. 72%, 40. 56%, 38. 33% and 34. 17%. In baits respectively in surely the ethanol extract of the nymph and the elder nymph feces and killing activity of significant progress, the nymph trapping consequences than the elder nymph. 目录: |
