| （摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 为了探究和控制德国鸢尾在北京天然气象前提下花芽分化的纪律，为完成其花期调控供给适用的参考材料，本文作者以德国鸢尾两季花种类‘常春黄’（Iris germanica cv．LovelyAgain）为研究资料，经由过程对其发育进程中的形状学不雅察，和内源激素、可溶性糖和卵白质含量静态变更的测定，初步控制了其花芽分化时代的形状变更特点和心理生化变更纪律。作者以德国鸢尾‘常春黄’为资料，运用扫描电镜（SEM）不雅察了德国鸢尾‘常春黄’的花芽分化进程，成果注解：德国鸢尾‘常春黄’的二次花花芽分化开端于5月底6月初，全部形状分化进程可分为6个阶段：花序原基分化期、外轮花被分化期、雄蕊分化期、内轮花被分化期、雌蕊分化期、胡须构成期，内轮花被片的产生和发育严重滞后于外轮花被片，德语论文，乃至晚于雄蕊的产生，这类花器官产生形式极其罕有。德国鸢尾‘常春黄’的花芽分化年夜概在50天阁下的时光完成；胡须的发育成熟是花蕾行将开放的主要标记。联合形状分化进程，分离取德国鸢尾‘常春黄’二次花花芽分化时代的顶芽、根茎和叶片部位，以酶联免疫吸附测定法（ELISA）测定内源激素的静态变更，对花芽分化期内源激素ZR（玉米素核苷）、IAA（吲哚乙酸）、GA3（赤霉素）和ABA（零落酸）含质变化停止了研究，成果注解：在花芽孕育临界时代内，随时光推移，叶片中IAA含量降低，ZR和ABA含量上升，GA3含量根本坚持稳固，顶芽和根茎由于部位的特别性变更情形与叶片中比拟有必定的差别，个中顶芽中的内源激素变更更加激烈。在形状分化不雅察和内源激素测定的基本上，以蒽酮比色法测定可溶性糖，以考马斯亮蓝G一250法测定卵白质含量，成果注解：可溶性糖在花序原基分化的初始阶段含量最高，且在三个部位的含量年夜小关系一直是：根茎》叶片》顶芽；卵白质含量呈先上升后降低的趋向，卵白质含量的峰值涌现于花序舒展早期，卵白质与可溶性糖之间存在必定的均衡关系。从德国鸢尾‘常春黄’的花芽分化进程及其花芽分化期两种重要代谢产品含量的静态变更纪律剖析，在华北地域若是在栽培办法方面改良植物的养分程度，进步德国鸢尾两季花种类‘常春黄’的花芽分化率，德语论文范文，其最好施肥时代为5月中下旬。而从内源激素的静态变更纪律剖析，要采用喷施外源激素到达二次花花期调控的目标，最好的喷施时代为5月下旬。 Abstract: In order to explore and Iris germanica control in Beijing natural weather conditions flower bud differentiation of discipline, to supply its flowering time regulation applicable reference materials for the completion of, the author of Iris germanica season two kinds of flowers' Changchun yellow '(Iris germanica cv.LovelyAgain) as the research data, by the process of its development in the process of the shape not Yacha, and endogenous hormones, soluble sugar and protein content dynamic changes of determination, the initial control of the shape of the age of the flower bud differentiation change characteristics and physiological and biochemical change discipline. Author to Iris germanica 'Changchun yellow' data, using scanning electron microscopy (SEM) indecent examine the Iris germanica 'Changchun yellow' flower bud differentiation process. As a result, note: Iris germanica 'Changchun yellow' secondary flower bud differentiation began in late May early June, all of the shapes in the differentiation process can be divided into six stages: inflorescence primordium differentiation phase, outer perianth differentiation stage, stamen differentiation stage, perianth initiation, pistil differentiation phase, beard constitute a period, tepals production and development is seriously lagging behind in outer tepals, and later than the stamens, the floral organ generated in the form is extremely rare. The eve of the differentiation of flower bud of Iris germanica 'Changchun yellow' almost in the 50 days of your time; maturation of the beard is the main marker of the bud to open. The joint shape differentiation process, the separation of the German iris' Changchun yellow 'two flower bud differentiation era bud, rhizome and leaf position, by enzyme-linked immunosorbent assay (ELISA) determination of static changes of endogenous hormones on endogenous hormones during flower bud differentiation, ZR (zeatin riboside (IAA), indole acetic acid), GA3 (GA) and ABA (and acid) content change of the study results, note: in the critical period of flower bud induction, as time goes on, reduce the content of IAA in leaves increased ZR and ABA content, GA3 content of root and rhizome buds keep stable, due to the special situation and the change of leaf position must match the difference of endogenous hormones in the bud change is more intense. Based on shape differentiation not Yacha and endogenous hormones determination, by anthrone colorimetry determination of soluble sugar, protein content was determined by Coomassie brilliant blue G 250 method, results of annotation: soluble sugar in the inflorescence primordium differentiation initial stage of content was the highest, and in three parts containing a large number of small relationship has always been: Rhizome leaf "bud"; protein content was first increased and then decreased trend, protein content of peak emergence in the inflorescence stretch early, protein and soluble sugar between exist certain equilibrium relationship. From static change discipline of Iris germanica 'Changchun yellow' the process of flower bud differentiation and flower bud differentiation stage of two important metabolic product content analysis, in the North China region if in cultivation measures on improvement of plant nutrient level and progress of Iris germanica season two kinds of flowers' Changchun yellow flower bud differentiation rate and the best fertilization age for mid to late May. And from the analysis of the static change of endogenous hormones, to the use of exogenous hormones to reach the goal of the two flower flowering regulation, the best spraying time is late May. 目录: |
