德国小蠊对杀虫饵剂的抗药性监测措施及行为选择探讨[德语论文]

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国小蠊是平常生涯中一种主要的卫生益虫,今朝在我国各地都是优势伤害种群。今朝,化学防治中的毒饵是掌握德国小蠊的重要办法。跟着新型毒饵的运用和抗性的发生,很有需要对毒饵的两年夜构成成份—卫生杀虫剂和基饵停止研究,以懂得抗性的发生除与杀虫剂自己的选择压力有关外,能否与德国小蠊灵敏的嗅觉、触觉等感触感染器对杀虫剂的感知,进而发生回避行动有关及饵剂的某些组分对德国小蠊取食行动的作用,同时监测德国小蠊分歧种群对经常使用毒饵杀虫剂的抗性及行动选择。研究成果可初步懂得行动在德国小蠊抗药性发生中所起的感化,既可以或许指点毒饵杀虫剂的迷信公道运用,又可以拓展德国小蠊的抗药性机制研究思绪,为德国小蠊的防治供给管理战略。1、德国小蠊若虫龄期差别的体征研究取德国小蠊的单卵荚停止豢养,每日不雅察并记载其蜕皮和龄期发育进度,测定若虫的头宽、体长及体重等生物学特点,经由过程统计剖析其重要体征值来肯定若虫龄期。成果注解:在绝对稳固的前提下,分歧龄时代的体重变更最显著,其次是体长。是以可依据体重、体长和头宽,对个别的龄期停止综合断定。在豢养前提下,德国小蠊若虫共阅历6个龄期,德国小蠊的龄期与头宽、体长或体重间呈正相干,且差别有统计学意义;相干系数(r)分离为:0.9539、0.9854、0.9879。2、德国小蠊对胃毒剂抗性测定的前提和基饵以德国小蠊雄成虫为试虫,测定光照、食品的几何地位和饥饿水平对食品选择的作用;以分歧发育阶段和性其余德国小蠊为试虫,以取食次数和逗留时光为目标,分离挑选,取其爱好的食品为基饵。成果注解,弱光对其雄成虫的取食有益;雷同前提下,食品的几何地位对雄成虫的取食无明显作用;将低龄(L1/L2)、高龄(L4/L5)和牝牡成虫分离饥饿2d比不饥饿、饥饿1天和饥饿3d,对食品的选择差别都显著,饥饿2d测定食品的差别较好,对食品的选择性由高到低的次序为:面包、猪肉松>奶粉>鼠料、饼干>麦芽糖>年夜豆粉>番茄酱、小麦粉。是以,可以选择猪肉松或面包为基饵,展开胃毒剂的抗药性监测。3、分歧种群德国小蠊对氟虫腈和乙酰甲胺磷的敏理性研究采取胃毒法测定分歧饥饿状况下我国分歧地域德国小蠊种群对经常使用杀虫剂推举剂量的LT5o及对罕见杀虫剂的LC5o,成果注解:江苏种群在不饥饿前提下对猪肉松1%乙酰甲胺磷饵剂表示出显著的敏理性,济南种群在饥饿前提下对面包表示出显著的敏理性;江苏种群和济南种群在不饥饿的前提下对0.05%氟虫腈猪肉松饵剂表示出显著的敏理性,但在饥饿前提下,试验室种群选择猪肉松饵剂;试验室种群、江苏种群和济南种群的德国小蠊在饥饿和不饥饿的前提下以面包和猪肉松为基饵的1%乙酰甲胺磷表示出显著的敏理性差别,且在不饥饿的前提下表示出强的耐受性;只要济南种群在饥饿和不饥饿前提下对0.05%氟虫腈面包饵剂没有表示出敏理性差别,其它情形下均表示出显著敏理性差别;氟虫腈饵剂抗性成果显示:以猪肉松为饵剂,试验室种群、江苏种群和济南种群的LC5o和LC95均无明显性差别;以面包为基饵,江苏种群和济南种群的毒力无差别;乙酰甲胺磷饵剂抗性成果显示:江苏种群和济南种群的毒力无差别,而且以猪肉松为基饵的毒性更高。4、分歧种群德国小蠊对经常使用杀虫剂的行动选择在室内用根本的天然选择法不雅察记载江苏和济南种群对经常使用杀虫剂氟虫腈、乙酰甲胺磷和溴氰菊酯推举剂量饵剂的行动趋势性,成果发明:江苏和济南种群的德国小蠊,不论是面包照样猪肉松为基饵,外行为选择上更趋势于含有乙酰甲胺磷的毒饵,且差别明显且趋势于不含有溴氰菊酯的毒饵,且差别明显;但两种地舆种群对0.05%氟虫腈的行动反响不显著,可口性和引导力不同一5、德国小蠊对杀虫饵剂的抗药性监测办法德国小蠊对杀虫饵剂的抗药性监测办法的前提为:试虫:肢体齐备、安康生动的雄成虫;饥饿处置:2天;试验前提:温度(26±1)℃,绝对湿度(60±5)%;光照:弱光,光照度分歧;基饵:猪肉松、面包。办法:胃毒法(以制备0.05%氟虫腈面包饵剂为例):秤取面包5g,在研钵中碾碎,用硫酸纸研匀。在面包上滴加5mg/mL(0.5%)的氟虫腈0.5mL,留意滴加平均,搅拌平均,阴干便可。

Abstract:

The German cockroach is a major health as daily life, now is the advantage of population in our country. At present, chemical control is an important way to grasp the bait of Blattella germanica. Along with the application and resistance of new type of bait, very need to bait the eve of the two constitute a component of hygienic insecticides and bait base research, except with insecticide selection pressure on the outside, whether with German cockroach sensitive sense of smell, touch feeling infection is to insecticides perception to understand the resistance, thereby avoiding action related and bait some group divided into impact on the feeding action of Blattella germanica, while monitoring the German cockroach, Blattella germanica population divergence of frequently used bait insecticide resistance and action selection. Research results can preliminary understand action in Blattella germanica anti drug action can perhaps pointing bait insecticide scientific and reasonable application, and can expand the Blattella germanica resistance mechanism research ideas for Blattella germanica control supply management strategy. 1, German cockroach nymphs age difference for signs of research from Blattella germanica single egg pods stop captive, daily observations and recorded the molting and age development progress, determination of nymphs of head width, body length and weight etc. biological characteristics, through statistical analysis of the important sign value to determine the nymphal instars. Results: in the absolute stability of the premise, the difference between the age of weight change is the most significant, followed by the body length. According to body weight, body length and head width, it is concluded that the individual. In captivity under the premise, German cockroach nymphs were experience six age and Blattella germanica age and head width, body length or weight were positively related to, and the difference is statistically significant; the correlation coefficient (R) separation for: 0.9539, 0.9854, are 0.9879. 2, Blattella germanica to stomach poison resistance determination of the prerequisite and basis of the bait to Blattella germanica male adults for testing insect, determination of light and food of the geometric position and hunger level influences on food choice; in different development stage and of the rest of the German cockroach, Blattella germanica to test insect, to feeding times and stay time, separating and selecting, take the love of food base bait. Results annotations, weak light on male adult feeding beneficial; similar premise, the geometric position of food fed to male adults without obvious effect; young (L1 / L2), aged L4/L5 and male and female adult hunger 2D than not hungry, starved for 1 day and hunger 3D separation, on food choice differences are significant, hunger 2D determination of food difference better, selectivity of food from high to low in order for: bread, pork and pine > milk > mouse feed, biscuits > malt sugar > Nianye flour > tomato sauce, wheat flour. So can choose pork floss or bread as bait, launched the stomach poison resistance monitoring. 3, differences between the populations of Blattella germanica to fipronil and acephate sensitivity study to stomach toxicity determination of differences in China under the hunger situation in different regions of Blattella germanica populations for regular use of insecticides recommended doses of LT5o and LC5o on rare pesticides, results note: Jiangsu population is not under the premise of hungry pork floss 1% acephate bait show significant sensitivity, under the premise of hunger in the Ji'nan population showed significant sensitivity of bread; Jiangsu and Ji'nan population without starvation of 0.05% fipronil baits showed loose pork sensitivity significantly, but the hunger condition, laboratory population pork bait agent; laboratory population, Jiangsu population and Ji'nan population of Blattella germanica in the premise of hunger and not hungry with bread and pork floss showed significant sensitivity for 1% acephate bait Pine; no bread and hunger in the premise of difference, and in not hungry expressed strong tolerance; as long as the Jinan population not hunger under the premise of 0.05% fluorine fipronil bait agents did not show the sensitivity difference and other circumstances have said a significant sensitivity difference; fluorine fipronil bait agent resistance results show: the pork floss for bait agent, laboratory population, Jiangsu population and Jinan population which and lc95 had no significant difference; with a bread based bait, Jiangsu population and Jinan population virulence difference of acephate bait agent resistance results show: Jiangsu population and Jinan population virulence indifference, and pork based bait more toxic. 4, differences between the populations of Blattella germanica to often use pesticides action selection in the laboratory using natural selection method of fundamental observation records of Jiangsu and Ji'nan populations of action tendency often use insecticide fipronil, acephate and deltamethrin recommended dose of bait, results of invention: Jiangsu and Ji'nan populations of German cockroaches, no matter bread still pork floss based bait, behavior choice are more likely to contain acephate bait, and the difference is obvious and the trend to do not contain deltamethrin baits, and the difference is obvious; but the two farming population to 0.05% and fipronil action response is not significant, palatability and guiding force of different resistance, 5 monitoring of insecticide to insecticidal bait of the German cockroach Blattella germanica to insecticidal bait resistance monitoring measures the premise for insects: Body: complete and vivid Ankang male adults; disposal of hunger 2 days; experimental conditions: temperature (26 / 1), absolute humidity (60 / 5)%; light: weak

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