(摘要内容经过系统自动伪原创处理以避免复制,下载原文正常,内容请直接查看目录。) 德国小蠊(Blattella germanica)是今朝蜚蠊中抗药性最强,最难防治的世界性城市卫生益虫之一。在生物防治中,运用自然源物资是一个极有开辟潜力的研究范畴。本文以德国小蠊为供试虫豸,经由过程比较实验分离挑选出毒饵载体、杀虫活性较好的年夜型有毒真菌及分歧处置产品、然后再停止年夜型有毒真菌复合制剂最好配比的挑选,终究肯定出对德国小蠊鸩杀后果较好的年夜型有毒真菌复合配方。研究成果以下:(1)经由对8种毒饵引导剂的挑选,在144h内德国小蠊对鼠饲料的摄取食量最高且稳固,再加上其成份多样化,本钱昂贵,是以将其作为毒饵载体。然后经由对4种增效辅料的挑选,得出参加5%红糖的后果较好,德语论文范文,肯定终究的毒饵载体为参加5%红糖的鼠饲料。在对德国小蠊豢养进程中发明,德国小蠊对高密度种群具有较强的抵御性,并且水对蜚蠊每一个发展阶段的感化尤其主要。(2)在年夜型有毒真菌对德国小蠊鸩杀实验中,起首将收集到的19种毒菌停止了菌种的分别与纯化造就,然后运用胃毒法将19种毒菌的野生籽实体和室内造就产品对德国小蠊停止鸩杀实验,得出残托斑鹅膏菌、鳞柄白毒鹅膏菌、豹斑毒鹅膏菌、条纹鹅膏菌、橙黄硬皮马勃的液体造就产品杀虫后果较好。对以上5种毒菌分离停止五个浓度梯度实验,得出LCso为残托斑鹅膏菌9。22480mg/g》鳞柄白毒鹅膏菌12。30383mg/g》豹斑毒鹅膏菌16。40019mg/g》橙黄硬皮马勃20。80282mg/g》条纹鹅膏菌23。22490mg/g。(3)在年夜型有毒真菌杀虫毒饵稳固性的实验中,分离对毒饵停止低温和强光照处置,然落后行杀虫实验得出,毒饵热贮稳固性较强,而光照稳固性较差。(4)从单剂当选出毒效较好的残托斑鹅膏菌、鳞柄白毒鹅膏菌、豹斑毒鹅膏菌三种毒菌,运用交互测定法停止最好配比的挑选,德语论文范文,结合毒力感化较强的三种复配措施的共毒系数分离为残托斑鹅膏菌/豹斑毒鹅膏菌143。98》残托斑鹅膏菌/鳞柄白毒鹅膏菌130。09》鳞柄白毒鹅膏菌/豹斑毒鹅膏菌124。55,表示为增效感化。半致逝世中浓度LC50为残托斑鹅膏菌/豹斑毒鹅膏菌7。89112mg/g》残托斑鹅膏菌/鳞柄白毒鹅膏菌7。95337mg/g》鳞柄白毒鹅膏菌/豹斑毒鹅膏菌11。18759mg/g。 Abstract: Blattella germanica is currently the cockroach in resistance to the strongest, one of the most difficult to control the world city health beneficial insects. In biological control, the use of natural resources is a very open up potential research areas. This paper to Blattella germanica was tested insects. Through comparison of experimental separation pick bait vectors, insecticidal activity better large poisonous fungi and different processing products, then stop large poisonous fungi compound preparation the best ratio of selection, eventually must be consequences better Nianye type of Blattella germanica poison to kill poisonous fungi compound formula. Research results as follows: (1) through the eight guide bait agent selection, within 144H Blattella germanica on rat feed intake of food intake highest and stable, plus the compositionally diverse, low cost is to the bait vectors. Then through the selected 4 kinds of synergistic accessories, it is concluded that the consequences of 5%, brown sugar, suitable for, certainly eventually bait vectors for brown sugar 5% in the feed. In of Blattella germanica in captivity in the process in the invention and Blattella germanica on high density populations with strong resistance and water effect on cockroaches every stage of development, especially the main. (2) on the eve of the poisonous fungi of Blattella germanica poison to kill experiment, chapeau collected 19 kinds of germs stopped strains respectively and purified to create, is then applied stomach toxicity will be 19 poisonous species of wild seed entity and interior to create products of Blattella germanica to stop poison to kill the, that disability care spot goose anointed bacteria, scales handle Amanita Verna bacteria, leopard Amanita phalloides bacteria, striped goose anointed bacteria, scleroderma aurantium liquid makes insecticidal products have good effects. The above 5 kinds of germ separation stop five concentration gradient experiments, it showed that LCso was a disability care AMANITAE in 9 spot. "22480mg/g" scale stem white poisonous goose anointed bacteria 12. "30383mg/g" leopard spot poison goose anointed bacteria 16. 40019mg/g "orange sclerodermataceae 20. "80282mg/g" striped goose cream bacteria 23. 22490mg/g. (3) in large poisonous fungi insecticide bait stability experiment, separation of bait disposal of low temperature and light, although fall after insecticidal experiments, heat poison bait storage stability is strong, and light stability is poor. (4) from single agent when selected toxic effect better residual spot goose anointed bacteria, scales handle Amanita Verna bacteria, leopard Amanita phalloides bacteria support three toadstool using interactive method for determination of stop, the best ratio selection, combination of virulence effect strong three formulation method of CO toxicity coefficient separation for disability care spot goose anointed bacteria / leopard Amanita phalloides bacteria 143. 98 "residual" "" "" "" "" "" "" "" "" "" "" "" "" "" "" "" "". 09 "scale stem white poisonous goose cream bacteria / leopard spot poisonous goose cream bacteria 124. 55, expressed as a synergism. Half caused by the death of the concentration of LC50 for the residue of the rest of the cream of the cream of the fungus / leopard spot poison mushroom 7. "89112mg/g", "residual", "", "" "" "" "" "" "" "" "" "" "" "" "". "95337mg/g" scale handle white poisonous goose cream bacteria / leopard spot poisonous goose cream bacteria 11. 18759mg/g. 目录: |