| （摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 控制抗性程度，懂得抗性机制是有用管理德国小蠊抗药性成绩的条件。该文应用生物测定，生物化学及份子生物学办法，对德国小蠊抗性程度、抗性检测办法、乙酰胆碱酯酶、谷胱甘肽转移酶、羧酸酯酶、细胞色素P450活性与光谱特点、细胞色素P450基因等几个方面停止了体系研究，旨在从群体、个别和基因三个程度提醒德国小蠊抗性发生的机制，为德国小蠊的抗性管理供给实际根据。运用药膜法测定了北京4个野外品系对氯氰菊酯，德语论文，溴氰菊酯，二氯苯醚菊酯和敌敌畏的抗性程度。成果注解，4个野外品系均对4种杀虫剂发生了分歧水平的抗性，个中，与敏感品系比拟，H品系对二氯苯醚菊酯和敌敌畏的抗性系数分离为5。38倍和6。97倍（KT），而用点滴法测定，抗性系数则分离为22。17、64。32和27。03倍（LD）。与药膜法比拟，点滴法对二氯苯醚菊酯、溴氰菊酯和氯氰菊酯的抗性系数分离要凌驾3。20倍、4。64倍和4。83倍。注解点滴法能更加准确地检测出德国小蠊的抗性程度，是一种较幻想的抗性检测办法。对采自北京分歧地域9种德国小蠊野外品系乙酰胆碱酯酶（AChE）和谷胱甘肽转移酶（GSTs）活性的测定成果注解：分歧野外品系间AChE和GSTs活性出现差别。有4种品系可以使AchE和GSTs活性进步，个中，KFQ品系与敏感品系比拟，AChE的活性进步了1。57倍，德语论文范文，BYS品系使GSTs活性进步了2。33倍；有3种品系可以使AChE活性进步，而使GSTs活性降低；尚有1种品系使AChE和GSTs活性均降低。 Abstract: Control the degree of resistance, resistance mechanism is useful to understand the management of Blattella germanica resistance performance conditions. The use of bioassays, biochemistry and molecular biology method, of Blattella germanica resistance, resistance detection method, acetylcholinesterase, glutathione transferase, carboxylesterase, cytochrome P450 activity and spectral characteristics, cytochrome P450 gene stopped system research, aimed at from the group, individual and gene three degree remind the mechanism of resistance of Blattella germanica, for Blattella germanica resistance management provide a theoretical basis. Application of membrane method was established for the determination of Beijing four field strains of Cypermethrin, deltamethrin, permethrin and DDVP resistant degree. Results annotations, 4 field strains were of 4 kinds of pesticides occurred different level of resistance, medium, compared with the susceptible strain, H strain of permethrin and DDVP resistance coefficient separate 5. 38 times and 6. 97 times (KT), but with the drop method, the resistance coefficient is 22. 17, 64. 32 and 27. 03 times (LD). Compared with the membrane separation method, resistance coefficient of drip method in two permethrin and deltamethrin and cypermethrin to above 3. 20 times, 4. 64 times and 4. 83 times. Note drop method can accurately detect the degree of resistance of Blattella germanica, is an ideal way to detect resistance. Collected from different district of Beijing 9 kinds of Blattella germanica field strains of acetylcholinesterase (AChE) and glutathione transfer (GSTs) enzyme activity determination results note: divergence field strains of ache and GSTs activity difference. There are 4 strains can make AchE and GSTs activity progress, in which the KFQ product line and the sensitivity of the Department of comparison, AChE activity of the progress of the 1. 57 times, BYS product line to make the GSTs activity improved 2. 33 times; there are 3 lines can make AChE activity progress, and make the GSTs activity decreased; there are 1 kinds of products to reduce the AChE and GSTs activity. 目录: 封面 1-2 |
