| （摘要内容经过系统自动伪原创处理以避免复制，下载原文正常，内容请直接查看目录。） 德国小蠊（Blattella germanica）是罕见的室内益虫之一，可以或许携带细菌、病毒、寄生虫卵等多种病原体流传疾病；另外一方面德国小蠊的排泄物或逝世亡虫体可以或许招致机体过敏；同时其为杂食性虫豸，并且分散较快，给居平易近生涯带来极年夜未便。今朝重要采取化学药物防制德国小蠊，跟着化学药物的赓续运用，运用药物种类单一，用药频仍，德语论文，德国小蠊生涯世代又较短，其抗药性成绩日渐凸起，遭到普遍存眷。拟除虫菊酯是一种仿生类农药，因其具有疾速击倒，德语论文题目，杀虫效能高，在情况中低残留及对人畜等非靶生物低毒等特色被普遍用于农业益虫和卫生益虫的防制。今朝的研究注解虽然运用拟除虫菊酯防制虫豸奏效快，但其对虫豸发生抗性的速度也快。国际相干报道注解德国小蠊对其发生的抗性也已达几十倍。是以若何防制已发生抗性的德国小蠊，若何晚期监测抗性的发生已迫在眉睫。对其抗性机理的研究发明，除代谢抗性外，击倒抗性（knockdown resistance，kdr）也是一个重要的抗性机理。份子生物学研究注解kdr表型与虫豸钠通道卵白ⅡS6—ⅡS6区亮氨酸被苯丙氨酸的替换有关。但是，国际在这方面的研究还没有见相干报道。是以对kdr展开相干研究不只有益于懂得我国该抗性的特点，而且可为晚期监测抗性发生及研究抗性的发生和成长，公道运用现有杀虫剂供给优越的实际基本。本研究取得的成果以下：1．对在北京部门地域现场收集的德国小蠊分歧品系用果酱瓶药膜法停止抗性测定。收集到的JFX株、AHQ株、XCL株、LFB株、DTL株和JYX株抗性系数RR50分离为2。13、3。86、6。31、8。70、5。07和3。36，均年夜于2，注解北京部门地域德国小蠊野外品系曾经对氯菊酯发生分歧水平抗性，个中XCL株、LFB株抗性系数较高。德国小嵘对氯菊醋击倒抗性与基因频率关系的研究中文摘要2。运用抗性较高的LFB株个别停止钠离子通道n 54一nS6区击倒抗性基因片断的RT一PCR扩增、克隆和测序，取得了钠离子通道n 54一n 56区部门序列片段，片断长度为162bP，经与GeneBank中德国小镰钠通道序列和试验室养殖的敏感株序列比拟，证明存在L993F渐变，该渐变是惹起击倒抗性的典范点渐变。注解在北京地域的德国小镰LFB株中存在击倒抗性。3。树立了用于德国小镰击倒抗性点渐变检测的等位基因特异 PCR法（PcRa功LPlification of speeifie allele，PASA）。成果显示德国小镰LFB株（40个别）和JFX株（47个别）中RR个别分离为5，0；55个别分离为22，41；RS个体分离为13，6。抗性频率RR、RS、55比例分离为1 2。5%和0、32。5%和12。8%、55%和87 。2%，抗性基因频率分离为2。88%和0。07%。 Abstract: Blattella germanica is one of the rare indoor pests, may carry bacteria, viruses, parasites and pathogens transmitted diseases; on the other hand of Blattella germanica excrement or died dead worms may lead to the body's allergic; at the same time, the omnivorous insects and disperse rapidly, to habitat civilian career brought huge inconvenience. Current important take chemical control Blattella germanica, follow the ceaseless application of medicinal chemistry, drug use single species, frequent medication, career generation of Blattella germanica and shorter, the achievement of resistance have become increasingly prominent, was widespread concern. Quasi pyrethrin is a kind of bionic pesticides, because of its rapid knockdown, high insecticidal efficiency, in case of low residue and of humans to non target organisms, toxicity and other characteristics is widely used in the prevention and control of agricultural pests and health beneficial insects. The current study notes although pyrethroid control insects rapid application, but its resistance to insect occurs faster. The report notes the coherent Blattella germanica resistance to its occurrence has reached dozens of times. So how to control the resistance of Blattella germanica has occurred, how advanced monitoring resistance has occurred imminent. The resistance mechanism of the invention, in addition to the metabolic resistance, resistance KDR (knockdown) is also an important resistance mechanism. Molecular biology research notes KDR phenotype and insect sodium channel protein II S6 - leucine II S6 region is replacing the phenylalanine. However, the international research in this area has not yet seen the relevant reports. Is to expand on the kdr coherent discussion not only to understand the characteristics of the resistance and advanced resistance monitoring and research resistance occurrence and development, the reasonable use existing pesticides supply superior. This research obtained the following results: 1. The sector region in Beijing on site collection of German Blattella germanica in the different strains with the pesticide smeared jam bottle method stop resistance determination. The resistance coefficient RR50 of JFX strain, AHQ strain, XCL strain, strain, LFB strain, DTL strain and JYX strain were separated into 2. 13, 3. 86, 6. 31, 8. 70, 5. 07 and 3. 36, the eve of the 2, annotations to Beijing Department regional Blattella germanica field lines once to permethrin occur the different level of resistance, medium xcl line, LFB resistant coefficient is higher. The study of German Chinese Rong down and resistance gene frequency of chlorine vinegar 2 chrysanthemum. Knockdown LFB application of higher resistance in the strains of individual sodium ion channel n a 54 nS6 area resistance gene fragment of RT PCR amplified, cloned and sequenced, and achieved n of sodium ion channel 54 n 56 district departments sequence fragments, fragment length 162bP. By compared with genebank in Germany small sickle sodium channel sequence and laboratory culture sensitive strain sequence, that there is a gradual L993F, the gradient is caused by knockdown resistance model of point gradient. Note in the Beijing area of German small sickle LFB strain in knockdown resistance. 3. Set for German small sickle knockdown resistance point gradient detection of allele specific PCR (PcRa work LPlification of this paper allele, Pasa). Results show that (40 individual) and the JFX strain (separate the individual 47 individual) RR 5, 0; 55 individual separated into 22, 41; RS individual separation for 13, 6 strains of German small sickle LFB. The frequency of resistance of RR, RS and 55 were separated into 12. 5% and 32, 0. 5% and 12. 8%, 55% and 87. 2%, the frequency of the resistant gene was 2. 88% and 0. 07%. 目录: |
