论文摘要:
Aeromicrobium panaciterrae (略)161~T和Microlunatus ginsengisoli Gsoil 633~T是从Pocheon参田分离出(略)兰氏阳性菌.为了分离这(略),做了多项实验.基于16S rRNA基因序列,Gsoil 161~T属于Nocardioidaceae家族,与它最为接近的菌株为Aeromicrobium marinum(98.0%类似度),Aeromicrobium alkaliterrae(97.6%),Aeromicrobium fastidiosum(97.0%)(略)microbium erythreum(96.7%).除上诉这些菌株之外,在Nocardioidaceae家族里,Gsoil 161~T的基因序列与其它种菌株的类似度都低于94.4%.而Gsoil 633~T属于Propionibacteriaceae家族,与它最为接近的菌株为Microlunatus phosphovorus DSM(略)~T,其类似度为96.1%.在Propionibacteriaceae家族里,Gsoil 633~T基因序列与其它... Two Gram-positive and strictly aerobic(omitted) (Aeromicrobium panaciterrae Gsoil 161~T and Microlunatus ginsengisoli Gsoil 633~T) were isolated from soil of a ginseng field i(omitted)Province, South Korea. The novel(omitted)were characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S r(omitted)equence similarity, strain Gsoil 161~T was shown to belong to the family Nocardioidaceae and was related to Aeromicrob(omitted)m (98.0% similarity to the type ... 目录:摘要 第3-4页 ABSTRACT 第4页 CHAPTER 1 INTRUDUCTION 第7-13页 ·Aeromicrobium and Microlunatus 第7-9页 ·Introduction of Bacterial Systematics 第9-13页 ·Classical phenotypic classification 第9-10页 ·Numerical taxonomy 第10页 ·Chemotaxonomy 第10-11页 ·Molecular systematics 第11-12页 ·Polyphasic taxonomy 第12-13页 CHAPTER 2 MATERIALS AND METHODS 第13-20页 ·Isolation of bacterial strains 第13-14页 ·Phenotypic analysis 第14-15页 ·Light Microscopy(purity, shape, size and motility) 第14页 ·Gram staining, Oxidase and Catalase test 第14页 ·Temperature and pH test 第14页 ·Salinity test 第14页 ·Nutrient utilization as sole carbon sources and enzyme tests 第14-15页 ·Anaerobic growth 第15页 ·Denitrification 第15页 ·Oxidation/Fermentation test 第15页 ·Determination of other characteristics 第15页 ·Chemotaxonomic studies 第15-17页 ·Respiratory isoprenoid quinone analysis 第15-16页 ·Fatty acid methyl ester analysis 第16页 ·Cell wall amino acids analysis 第16页 ·Polar lipid analysis 第16-17页 ·Molecular Taxonomy 第17-20页 ·DNA extraction 第17页 ·Determination of G/C content 第17-18页 ·S rRNA gene sequencing and phylogenetic analysis 第18页 ·Nucleotide sequence accession numbers 第18-20页 CHAPTER 3 RESULTS AND DISCUSSION 第20-35页 ·Aeromicrobium panaciterrae Gsoil 161~T 第20-28页 ·Isolation and morphology 第20页 ·Physiological and biochemical characteristics 第20-21页 ·Chemotaxonomic characteristics 第21页 ·Phylogenetic analysis based on 16S rRNA gene sequences 第21-27页 ·Description of Aeromicrobium panaciterrae sp.nov. 第27-28页 ·Mierolunatus ginsengisoli Gsoil 633~T 第28-35页 ·Isolation and morphology 第28页 ·Physiological and biochemical characteristics 第28-29页 ·Chemotaxonomic characteristics 第29页 ·Phylogenetic analysis based on 16S rRNA gene sequences 第29-33页 ·Description of Microlunatus ginsengisoli sp.nov. 第33-35页 REFERENCES 第35-39页 PUBLICATIONS 第39页 Presentation 第39-41页 致谢 第41-42页 APPENDIX 第42-45页 |